Microfluidic systems have major advantages in studying biological phenomenon since they can mimic aspects of the 3D in vivo situation in a controlled environment while simultaneously providing in situ imaging capabilities for visualization and enabling cell-cell and cell-matrix interaction quantifications. Despite supporting experimental evidence showing the importance of complex microenvironment, none of the previously reported in vitro systems has reproduced the specific cross-talk among several cell types in a complex pathophysiological microenvironment such as cancer or atherosclerosis model. Moreover, microfluidics allow parametric study of multiple factors in controlled and repeatable conditions. In Biomicrofluidics Lab, we will develop a microfluidic assay and use it to study different disease models including cancer metastasis and evolution of atherosclerosis. The platform will allow organ-specific mimetic to better clarify the mutual interactions between different cell populations in a well-defined microenvironment, in order to develop highly focused and more effective therapies.